Перейти в головне навігаційне меню Перейти до головного Перейти на нижній колонтитул сайту

Біологія та біотехнології

August 18, 2023; Cambridge, UK: V Міжнародна науково-практична конференція «EDUCATION AND SCIENCE OF TODAY: INTERSECTORAL ISSUES AND DEVELOPMENT OF SCIENCES»


THE EFFECT OF A MIXTURE OF SELECTED NANOPOLLUTANTS ON THE LEVEL OF DNA DAMAGE IN THE BLOOD OF CYPRINUS CARPIO FRY


DOI
https://doi.org/10.36074/logos-18.08.2023.28
Опубліковано
29.08.2023

Анотація

Chemical compounds, widely produced and released into aquatic ecosystems, pose threats to biocenosis and accumulate within food chains. This study examines the impact of four specific pollutants – ibuprofen, SDS, EE2, and DMSO on DNA damage in nine-month-old Cyprinus carpio fry. These pollutants are detected in groundwater, surface water, and drinking water. The study employs the Single Cell Gel Electrophoresis (Comet Assay) to analyze DNA damage levels resulting from exposure to the pollutants. The chosen fish species, serves as a suitable model due to its resilience to diseases and exposure to mutagenic substances. Results indicate a dose-dependent reduction in DNA content in response to pollutant exposure. This research highlights the urgent need to assess the genetic impact of pollutants on aquatic ecosystems and emphasizes the importance of understanding the risks posed by these contaminants.

Посилання

  1. Song, B., Zeng, G., Gong, J., Liang, J., Xu, P., Liu, Z., Zhang, Y., Zhang, C., Cheng, M., Liu, Y., Ye, S., Yi, H., & Ren, X. (2017, August). Evaluation methods for assessing effectiveness of in situ remediation of soil and sediment contaminated with organic pollutants and heavy metals. Environment International, 105,
  2. –55. https://doi.org/10.1016/j.envint.2017.05.001
  3. Rahman, M. M. (2015, May 22). Role of common carp (Cyprinus carpio) in aquaculture production systems. Frontiers in Life Science, 8(4), 399–410. https://doi.org/10.1080/21553769.2015.1045629
  4. Sobrino-Figueroa, A. S. (2013, August). Evaluation of oxidative stress and genetic damage caused by detergents in the zebrafish Danio rerio (Cyprinidae). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 165(4), 528–532. https://doi.org/10.1016/j.cbpa.2013.03.026
  5. Zheng, B., Li, W., Li, H., Liu, L., Lei, P., Ge, X., Yu, Z., & Zhou, Y. (2016, August 31). Separation and determination of estrogen in the water environment by high performance liquid chromatography-fourier transform infrared spectroscopy. Scientific Reports, 6(1). https://doi.org/10.1038/srep32264
  6. Organization, W. H. (2013, January 1). WHO model list of essential medicines : 18th list, April 2013. WHO Model List of Essential Medicines : 18th List, April 2013. https://apps.who.int/iris/handle/10665/93142
  7. Augustyniak, M., Orzechowska, H., Kędziorski, A., Sawczyn, T., & Doleżych, B. (2014, February). DNA damage in grasshoppers’ larvae – Comet assay in environmental approach. Chemosphere, 96, 180–187. https://doi.org/10.1016/j.chemosphere.2013.10.033